FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17516910
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG92100.018.0144085
GTGTAGG71650.017.0656831
CTATACT61800.016.0471864
GTATTAG55200.015.76155951
TACACTG95800.015.748695
GTATAGG35650.015.1706731
CCTATAC39150.015.0101423
CTAGTAC29000.014.5898593
GTATATA145700.014.3637231
TATACAG81200.014.3549135
TATACCG8550.014.2926295
TAGACAG101350.014.0515355
TCTATAC59250.014.0440223
TACACAG120650.014.0242645
GTCCTAG59550.013.978421
GTGTAGA77700.013.9210941
GTATAGA58550.013.8958711
GTCTTAG55600.013.871891
CTACACT73450.013.8218724
TAGACTG72150.013.7451985
Produced by FastQC (version 0.11.5)