FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17912087
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT61400.018.5980034
TATACTG91250.018.436355
GTATTAG50700.016.338471
GTATAGG41350.016.2767311
CCTATAC42300.016.2242473
TACACTG97000.016.083895
TATACAG82600.015.9864145
TCTATAC57550.015.9272723
CTAGGAC63950.015.72987653
GTGTAGG77850.015.05384351
GTATATA128900.014.9705461
TAGACAG96250.014.8915
TAGACTG76100.014.8818595
CTACACT70000.014.7019354
GTATAAG48750.014.5783711
TACACAG129000.014.5711915
TACACCG20050.014.2968455
GCTACAC58250.014.2833293
GTGTAGA84700.014.2809271
GTATAGA60350.014.193871
Produced by FastQC (version 0.11.5)