FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12595392
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG54500.018.7101335
GTATTAG37100.018.2533251
TTATACT61100.016.5352544
GTATTAT61750.015.7647391
TATACCG14450.014.9590365
GTATTAA52200.014.6848831
TACACCG25000.014.6611565
CTAGGAC36750.014.4488983
CTATACT44400.014.3936044
TAATACT62350.014.244234
TATACTA37600.014.1222615
CTAATAC37450.014.05334953
CTAGACT33400.013.9284364
GTATAGG31000.013.8048951
TCTATAC43200.013.7056743
ATAATAC61150.013.6784583
GTATATA79850.013.6636421
ATTATAC52900.013.5909373
CCTATAC32950.013.5482063
TACACTG65850.013.4157725
Produced by FastQC (version 0.11.5)