FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12595392
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN136260.1081824209996799No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG54100.018.0283955
CTATACT42550.016.2038964
GTATTAT59350.015.1821431
GTATTAG36600.014.6176081
TACACTG66900.014.4388325
GTATTAA53800.014.1314051
TATACAG59850.014.0242575
GTATAAG39450.013.7995071
ATAATAC61550.013.7183383
GTATATA77700.013.710631
GTGTAGG58750.013.659691
CTACACT50000.013.5080984
GTATTAC39350.013.4767851
GTATAGA42950.013.4398531
TCTATAC41500.013.3380073
TAGACTG48600.013.218295
GTGTAAG47850.012.9462681
CTAGACT36650.012.9255524
TTATACT64000.012.825074
TAATACT60450.012.802344
Produced by FastQC (version 0.11.5)