FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14090133
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG68100.017.0448785
GTATAGG29250.015.7479071
GTATAGA44000.015.0622241
TACACTG75850.014.9935285
TAGACTG54450.014.3269665
CTATACT47050.014.2830344
GTATAAG40950.013.8884621
GTGTAGG60150.013.8312131
GTATTAG45100.013.5484461
TCTATAC46200.013.4289093
GTGTTAG56500.013.3104931
GTATATA109900.013.2582641
TACACCG13400.012.9760055
TAGACAG81700.012.9420925
TAATACT63600.012.8568974
CTAATAC42500.012.8285583
TTATACT67100.012.8165934
CTACACT53450.012.7486544
TATACAG59500.012.7160715
GTATTAC37800.012.6832641
Produced by FastQC (version 0.11.5)