FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12605299
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA23300.022.58015894
TCGCCGT39650.021.562294
GTCGCCG54600.021.16446794
GGTCGCC73650.019.58080394
GCCGTAT19450.019.56276994
CTATACT33750.017.5412254
TGGTCGC83700.016.78072294
TATACTG51200.016.1514345
GTATATA69550.015.6160521
GTATAGG24200.015.5428631
TATACCG5205.456968E-1215.3608045
GTGGTCG104100.014.39477494
TTATACT61750.013.8483374
TCTATAC32950.013.8357253
GTATACG7150.013.8092361
GTGTATG58700.013.6165531
CCTACAC37450.013.5536913
CTAGGAC35300.013.4472123
TACACTG53550.013.3368265
GTGTAGG41550.013.2394891
Produced by FastQC (version 0.11.5)