FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14057169
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA20550.020.57863294
TCGCCGT37050.018.64296394
GGTCGCC65100.018.4053994
TATACTG68650.018.2040545
TACACTG74800.017.8379025
GTCGCCG50150.017.70829494
TGGTCGC75350.017.46065994
GTGTAGG57300.015.9278691
TATACAG66700.015.1439695
GTATAGG32300.015.0018651
CTATACT46000.014.9110474
CTACACT56650.014.8445234
CCTATAC32650.014.6768093
TAGACTG55050.014.5937155
GTCTAAG35800.014.5864811
CTAGACT45000.014.5116054
GTATAGA42000.014.1134061
GTCTTAG41450.014.073681
TACACAG93500.013.9688365
GCCGTAT19550.013.94013894
Produced by FastQC (version 0.11.5)