FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15013360
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG69750.017.519885
TACACTG81550.016.3680295
CCTACAC56300.016.363593
GTATAGG36550.016.2083931
GTATAGA47200.014.7426821
CTACACT60950.014.7286064
GTATAAG41650.014.2236921
TACACCG13250.014.1888165
GTATTAG46850.014.1503211
TCTATAC43700.014.0903193
TATACAG65450.014.0750015
TAGACTG58550.014.0479575
GTCCTAG47650.014.0114231
GTGTAGA65700.013.9548781
GTATATA106550.013.9440981
GTGTAGG64650.013.6724431
CTATACT48350.013.609234
GTGTATA74650.013.6044431
GTCTAGG48550.013.3643141
CTTACAC58450.013.1883453
Produced by FastQC (version 0.11.5)