FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15013360
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA14800.018.7249694
TATACTG70200.018.3399245
GTATTAG46750.016.5930711
GTATAGG32600.016.4403741
TACACTG78850.015.7320855
TAGACTG55850.015.4802765
TGGTCGC53800.015.19139394
CTATACT47650.015.0872374
GTCGCCG39450.014.88310794
GTATAAG42750.014.7364591
GGTCGCC51000.014.73602794
TATACAG65200.014.7016695
TCTATAC45650.014.6198073
TCGCCGT29050.014.22876394
CTACACT59450.014.1475864
GTATATA107400.014.0953561
GTGTAGG57900.014.0472721
GTCCTAG49350.014.0040811
CCTATAC32600.013.9845423
CTAGACT46450.013.959654
Produced by FastQC (version 0.11.5)