FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682533
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG42250.018.6882515
GTATACG8000.015.2783751
GTATAGG21300.015.2287161
GTCCTAG26750.014.5863991
GTGTAAG31900.014.4420621
TACACTG51800.014.2447895
CTAGACT26800.014.204844
TACACCG16550.014.1990125
GTGTTAG34700.013.954091
GTATTAC25500.013.8265831
ATACTGT60900.013.8140766
GTACTAG15150.013.653181
GTATTAG29150.013.5467321
GTCTTAG28550.013.5021071
CTAATAC28700.013.4290413
GTATATA58150.013.4199891
GTATAAG27400.013.3825181
CTAGTAC14400.013.3824133
TAGACAG49000.013.1404615
GTATAGA31900.013.115751
Produced by FastQC (version 0.11.5)