FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14390945
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG66400.016.9881365
TACACTG74250.016.521395
CTATACT48250.016.0727144
GTATTAG44900.015.7108461
GTATAAG40400.015.5983291
GTATAGG28800.015.1860611
TCTATAC44700.014.9344433
CTACACT56400.014.9168294
GTGTAGG53350.014.632831
GTATAGA46650.014.6174291
CCTACAC52650.014.5545063
GTATATA97800.014.3295351
TAGACTG55100.014.0745635
GTGTACG14100.014.0082811
GTCTTAG42700.013.9871911
TATACAG63850.013.9860575
CTAATAC45300.013.4912883
CCTATAC30900.013.38851453
GTCTAGG42200.013.3728331
GTGTTAG58150.013.1823411
Produced by FastQC (version 0.11.5)