FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14390945
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG41850.019.31189794
CGCCGTA17000.017.68980494
TATACTG64800.017.6885415
GTATAGG30450.016.214281
TCGCCGT30300.016.12807894
GTATAAG44050.015.6915951
GTATTAG45100.015.534791
TGGTCGC57550.015.43151194
CCTATAC27950.014.9668383
CTATACT46550.014.8345674
TAGACAG79950.014.7479775
TAATACG11150.014.7458694
TACACTG69100.014.6842875
GTGGTCG74650.014.54032594
GTCCTAG46050.014.3974351
GCCGTAT16000.014.39024194
GGTCGCC55450.014.23638394
CTAATAC44600.014.0164713
GTATATA98500.013.7006561
CTACACT54900.013.6906734
Produced by FastQC (version 0.11.5)