FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19057349
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG92600.018.0654775
GTATTAG54250.016.2123341
GTATAAG54800.016.1354471
ATTATAC78850.015.7331633
CTATACT63550.015.4540094
TTATACT92150.015.1450794
GTATTAT86800.014.7926721
TACACTG110900.014.6183375
TATACAG93600.014.5088315
CTAATAC57550.014.3708093
TAATACT87550.013.7402374
TCTATAC60050.013.6942693
CTACACT82650.013.588314
GTATTAA75450.013.3400751
GTATAGA62500.013.0940191
ATACTGT131850.013.0796226
ATAATAC84850.012.8484283
TAATACA109700.012.7649774
GTGTAGG91850.012.6991961
CTAGTAC31750.012.5802433
Produced by FastQC (version 0.11.5)