FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19057349
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN215800.11323715591292367No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG99000.015.8148925
TACACTG112600.015.3201975
GTATAGG46500.014.7370111
TATACAG92900.014.532215
GTATTAG55500.014.4614771
TGGTCGC58200.014.38345894
GTATAAG53100.014.31957151
CGCCGTA19900.013.70696394
CTATACT66900.013.5966254
TAATACT84700.013.5070944
ATACTGT136450.013.3263566
ATAATAC84600.013.2504133
GTATTAT85850.013.1760821
GTATAGA61750.013.1497861
GTGTTAG70650.013.0212731
GTATTAA71900.012.9254541
ATTATAC79450.012.8695823
TCGCCGT34250.012.63262394
TAGACTG78900.012.536045
CTACACT78250.012.5232614
Produced by FastQC (version 0.11.5)