FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18373162
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG83150.018.8211575
GTATAGG36500.016.759051
CTATACT60450.015.9375644
GTATAAG51550.015.7912451
TACACTG90850.015.6740825
CCTACAC64700.014.8208883
GTGTAGG69850.014.3486861
GTATTAG53500.014.3361571
TACACAG115500.014.3227055
TATACAG79550.014.0014355
CTACACT69050.013.952584
CTAGACT53700.013.8276124
GCTATAC33100.013.7750143
TTATACT93650.013.7501424
GTATAGA55200.013.7241591
TCTAGAC54950.013.6867583
GTATATA116500.013.4902331
TAGACTG69850.013.4563725
GTGTATA80600.013.4273951
CCTATAC38250.013.2721293
Produced by FastQC (version 0.11.5)