FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12848733
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG53000.018.43954894
TCGCCGT38650.018.2349594
CGCCGTA22150.017.60626294
TGGTCGC72750.017.05036794
TATACTG58000.016.6903845
CTAGACT37650.015.7263374
CTATACT42600.015.6639324
TAGACTG49300.015.5370215
GTCCTAG38000.015.4694521
GGTCGCC65400.015.23073894
CTAGGAC42000.015.2251043
TAGACAG66450.014.992295
TACACTG64250.014.7742455
GCCGTAT20450.014.47471694
CCTACAC47800.014.2629963
TACACCG13900.014.1991255
TCTATAC38900.014.0210023
CCTATAC28600.013.80968953
GTCTAGG37400.013.5800281
GTATAAG35350.013.5693571
Produced by FastQC (version 0.11.5)