FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14979918
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG60400.017.5086385
GTATAGG30600.015.3681561
CCTATAC31300.015.0200443
TATACAG63400.014.8268035
GTATATA94200.014.7271
CTATACG7300.014.1646764
CTACACT58850.014.0563644
GTACTAG24500.013.6281041
TACACTG71900.013.5969425
CTATACT45100.013.5479524
GTGTAGG52750.013.461631
GTATAGA42850.013.2793781
GTGTATA64550.013.2592311
GTATTAG42850.013.1696311
GTGTTAG57950.013.1463361
CTATACA50700.013.0712754
GTCTAGG41100.013.0438631
TAGACAG80300.012.99404055
CCTACAC52200.012.9690313
TCTATAC43750.012.8949213
Produced by FastQC (version 0.11.5)