FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11386205
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG50600.018.1104895
CGCCGTA22400.016.78275594
CCTATAC24500.016.3124943
GTATAGG27950.015.8240391
CTATACT39300.015.6643864
GTCGCCG45000.015.66390494
GTGTAGG50400.015.1236211
TACACTG60150.015.0788345
GTATTAG35900.015.0721331
GGTCGCC57000.014.83948894
TGGTCGC60550.014.8231594
GTACTAG18300.014.6553121
CTACACT46700.014.3897684
GTATAAG34550.014.2992261
GCTACAC36600.014.1312043
TACACCG15650.014.1133345
GTCTTAG34850.013.7711011
TACACAG72300.013.5198265
GTCTAGG35950.013.3497341
TCTATAC37750.013.0779573
Produced by FastQC (version 0.11.5)