FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18269662
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG56100.018.8433394
TCGCCGT40400.018.83960294
CGCCGTA22900.018.66998594
TGGTCGC83650.016.96207694
GGTCGCC75500.016.86398994
GTATAGG38800.015.6195971
GTGTATA76450.015.0556821
CTATACT59500.014.6847774
TATACTG74300.014.5419795
GTGTAGG63600.014.4780751
GTATATA107050.014.0434941
TACACTG87150.013.9071065
GTATTAG55300.013.8475951
TATACAG76950.013.79698855
CCTATAC40350.013.736443
GTGGTCG105100.013.63437294
GTATTAC50400.013.5160361
CTAGGAC57350.013.5140733
TAGACAG99100.013.36785
CCTACAC58500.013.1681183
Produced by FastQC (version 0.11.5)