FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19176835
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21200.018.5654531
GTATTAG41300.015.2145071
GTACTAA40700.015.0994841
GTTCTAG55900.014.9465211
GTCCTAG49950.014.8607071
GTCTTAG43050.014.1950411
GTATTAA60850.013.8441331
GTATATG59850.013.4985841
GCTCTAG52100.013.4522481
CTATACT47850.013.4203584
GTTCTAA57150.013.4113741
GTCCTAA39850.013.3422451
CCTATAC31900.013.0955023
TATACTG63150.012.9025055
GTATATA77200.012.7904331
GTGCTAG44650.012.7584821
GGTCTAG37750.012.71261
GTGTTAG51100.012.702041
TCTAGAC45550.012.6577223
GTCTATA36100.012.4329071
Produced by FastQC (version 0.11.5)