FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19176835
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG25150.018.5714551
GTCCTAG50850.017.2139191
GTATTAG39000.016.6779791
GTTCTAG52800.016.3815981
GTACTAA37700.015.6011911
GTATATA75950.014.6226961
GCTCTAG53200.014.3074171
GTGCTAG49050.013.8956021
GTATTAA59350.013.874151
GTCTTAG44200.013.7765521
GGTCTAA36150.013.6860431
TCTATAC44850.013.6301653
GTTCTAA59900.013.573481
TCTAGGC61450.013.376593
TCTGTAC84700.013.0076123
GGTCTAG38850.013.0020551
GTCTTAA51850.012.8115791
TATACTG59400.012.7263985
GTATTAT59250.012.6713091
GTACATA54900.012.6670041
Produced by FastQC (version 0.11.5)