FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18683540
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG39100.018.1861131
GTACTAG23050.017.9704821
GTCCTAG47050.016.3604281
CGATCGT7150.015.93455238
TCTATAC42200.015.0505933
GTATTAA60750.015.00054551
GCGATCG7600.014.99001537
TACGCGA3051.4586476E-814.7127182
GTACTAA37200.014.6609991
GTCTTAG43150.014.2392991
GTATAGG32550.013.7861121
GTGCTAG45450.013.518721
GTATATA69500.013.4596541
GTATAAG35650.013.458751
GTTCTAG54200.013.3105631
TCTGTAC79050.013.1435563
GCCCTAG45250.012.8156351
GGTCTAG35350.012.791791
GTACTAT36700.012.7915231
GTCTTAA51550.012.7225561
Produced by FastQC (version 0.11.5)