FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18683540
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG36350.017.7023621
GTACTAG24100.017.3696481
GTCCTAG48950.016.2554051
GCTCTAG54200.015.1276591
GTACTAA35650.015.0416271
GTTCTAG55250.014.4644651
GTATTAA58550.013.9446541
GTATAGA47800.013.8238221
GTGCTAG43300.013.742421
GTCTTAG43000.013.5164771
GTCCTAA41200.013.2673011
GTTCTAA56750.013.2286821
GTCTAGA42850.013.1601081
CGATCGT6900.013.00959438
GTATATG60700.012.9947771
GTGCTAA50200.012.88725951
GTCCTAT41200.012.8474491
GTATAGG32350.012.8330421
GCGATCG7050.012.73187337
TCTATAC42450.012.6925233
Produced by FastQC (version 0.11.5)