FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18694029
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG46750.018.39041
GTACTAG22950.018.053941
GTCCTAA41750.015.3826071
GTATTAA62050.015.3025691
GCTCTAG52700.015.0692171
GTATAGG31650.015.0549341
GTATTAG38400.014.8363191
GTACAGG82700.013.9031171
GTATATA72950.013.72606851
GTACTAA36950.013.5496171
GGTCTAG35800.013.5026331
TCTATAC40450.013.4013573
GTGCTAG45300.013.3386941
GTCTTAA51600.013.2491781
GTTCTAG56150.013.2209341
CTATACT42850.013.1342254
GTCTTAG43250.013.0129251
GTATAAG42050.012.7273841
TAGTGCG9400.012.65620357
GTACAAG64700.012.5945071
Produced by FastQC (version 0.11.5)