FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17767373
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21950.018.5558261
GTATTAG39550.016.058471
GTCCTAG46200.015.9883851
GTGCTAG43000.015.9741381
GTATTAA59300.014.493641
GTCCTAA39750.014.1541341
GTTCTAG51700.013.9536741
GTACTAA36700.013.9196551
GTGTTAG43150.013.5188181
GTACTAT34300.013.4847691
GTCTTAG41500.013.4740991
GCTCTAG51000.013.333031
GTATAGG30950.013.2714721
GTATATA69650.012.7859031
GTATAGA44450.012.7351751
GGTCTAA31550.012.6908721
GGTCTAG34800.012.5967141
CTATACT42850.012.4858274
TCTAGAC40350.012.3183633
TATACTG56650.012.247155
Produced by FastQC (version 0.11.5)