FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17767373
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG37750.017.7539231
GTACTAG20750.017.6480731
GTCCTAG46600.017.5700071
GTACTAA36900.017.1330341
GTTCTAG51050.015.4970581
GTGCTAG44000.015.07502651
GTCCTAA39300.014.6802491
GTCTTAG41000.014.5771211
GTATATA73200.014.5361431
GTATTAA56800.014.0498981
GCTCTAG50500.014.0240041
GGTCTAG34350.013.97969151
GTACAGG77600.013.9345251
GTTCTAA56100.013.4246561
CGATCGT7500.012.88870238
GTCTTAA48950.012.8448261
GTCCTAT41700.012.8411871
TCTATAC38700.012.6680733
GTATAGG29250.012.5195731
GTGCTAA43600.012.5193031
Produced by FastQC (version 0.11.5)