FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4425970
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA9150.017.7154081
GTTCTAG11200.017.2442531
GTACAGG20100.015.9573711
GTCCTAG9900.015.6765971
CTATACT8850.015.5887314
GTATTAG6950.014.8871271
TCTAGAC9400.014.6762933
GTATATA12950.014.647631
GTACTAA8100.014.4766591
GTCCTAT7900.014.4065911
GTACTAT8450.014.2851821
CTAGACG2001.988316E-413.7960274
GCTAGAC7750.013.7957143
GTATTAT10550.013.7300221
GTGCTAC10700.013.5375451
GTCTTAG9200.013.4955031
GTTCTAA10850.013.350391
GGATTAG9300.013.350391
TAGTGCG1850.001536810513.25762957
TATACTG11550.013.1390735
Produced by FastQC (version 0.11.5)