FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4425970
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7550.021.0287911
GTTCTAG11600.018.7450241
GTATTAA12150.017.6124111
GTACTAG5350.017.4186061
GTACTAA8750.017.3559381
CTCGCGT2057.8099583E-716.82024413
GCTCTAG12550.016.5010261
GTCCTAG10000.016.2218841
GTCCTAA9050.016.0178471
ATTAGAC7850.015.8142423
GGTCTAA7950.015.19512751
CTAGACT9300.014.8304174
GTGCTAG10900.014.5658141
GCGTAAG2403.9913866E-614.3811031
CTAGTAC4850.014.22013953
GGTCTAG10000.014.1510051
GTCTTAA10350.014.0059441
GTTCTAA12450.013.8613031
CTGTACT22650.013.8531824
CGCACTA1750.001139669313.7936931
Produced by FastQC (version 0.11.5)