FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4336597
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG8700.020.613771
GTATTAA11850.019.7908251
GTACTAG3850.018.8118671
GTATAGG6950.017.8645081
GTTCTAG11950.017.0277581
GGTCTAA7500.016.5544431
TTGCGCG1503.5413285E-416.09212
GTCCTAA9350.015.4921261
ATAACGC1808.1168444E-515.3281873
GTTCTAA11450.015.0604471
GTCCTAG10300.014.7329181
GTATATA13400.014.4130721
GCACTAG7000.014.288061
GTGCTAG9200.014.2452171
GTATTAT11650.013.9137841
GCCCTAG9850.013.6553141
TCTAGAC8600.013.6349563
TCTGGAC29300.013.5364113
GTGTTAG9800.013.3730621
GACTAGT3102.8580325E-713.3503571
Produced by FastQC (version 0.11.5)