FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10247949
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12100.019.3868681
GTCCTAG26750.016.7650011
CGATCGT3158.0035534E-1116.38242538
GTATACG4500.016.0987011
GTATTAG19550.016.0575281
GTCCTAA22650.015.8397831
GTTCTAA27900.015.7030351
GGTCTAG20450.015.182151
GTCTTAG21100.014.8779481
GCCCTAG25950.014.7560361
GTATTAA28350.014.6019951
GTACAGG42450.014.5465311
GTGCTAA23050.014.5172671
GGTCTAA18300.014.5152231
GTACTAT19050.014.3059331
GTTCTAG28800.013.7749311
GCGATCG3801.3624231E-913.57930637
GTACTAA17600.013.5244771
TCTATAC20900.013.1822883
GTCTTAA26900.012.9524881
Produced by FastQC (version 0.11.5)