FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10424542
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG23200.017.6434781
GTACTAG11800.017.490191
GTATTAG18450.017.3385121
GTCCTAG25850.017.1654191
GTGCTAG26700.014.1712021
GTATATA35050.013.9355961
GTCCTAA20850.013.8579341
GGTCTAG20650.013.8255791
GCTCTAG28750.013.7589491
TCTAGAC23350.013.405853
GTTCTAG29150.013.3341431
GTATTAA27650.013.3110991
ATAGTAC17350.013.2835813
GTTCTAA29550.013.2700521
CTAGACT22600.013.2419744
GTACATA23750.013.1796251
GTATTAT27650.013.0622941
GTCTTAA29650.012.7612511
GTATAAG21300.012.596221
GTATAAA38750.012.5162061
Produced by FastQC (version 0.11.5)