FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10424542
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13200.018.0336741
GTATAGG15250.017.1930471
GTCTTAG22850.016.305971
GTATTAG17250.016.1996271
GTCCTAG23200.015.911271
GTATTAA27600.015.124651
GTCCTAA20100.015.1041291
GTTCTAG25600.014.8238771
GTTCTAA30400.014.6394651
GTGCTAG25000.014.4896661
GTACTAA18650.014.2436391
TCTAGAC22250.013.4688413
GCTCTAG30000.013.454691
GTATATA34200.013.4163561
GTCTTAA27150.013.2151641
GTACATA26450.012.9127451
GGTCTAA21800.012.6602581
GCGCTAG3104.142774E-612.24165251
GCCCTAG24700.012.1515421
GTATTAT26700.012.1457861
Produced by FastQC (version 0.11.5)