FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357783
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12250.019.1134241
GTCCTAG27100.018.4231741
GTATTAG20150.017.9423751
CGATCGT3950.017.42261338
GTTCTAG28550.015.4372371
GTACTAT21500.014.7338091
GGTCTAA21100.014.6867521
GCTCTAG30650.014.6042271
GTATAGG17400.014.2478371
GCGATCG4600.014.21145137
GTACTAA17250.014.1721241
GCGCTAG3455.0513336E-913.9725171
TAGTACT20900.013.4965954
GTACGAA6400.013.4501081
GGTCTAG19350.013.3458421
GTCTTAG23350.013.271541
GTGCTAA21750.013.1396721
GTGCTAG23900.012.9661281
GTATAAA39600.012.9555771
GTTCTAA29650.012.77419951
Produced by FastQC (version 0.11.5)