FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10294791
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17800.019.7123411
GGTCTAA18100.018.2452871
GTGCTAG24200.016.9156881
GTCTTAG23000.016.1530281
GTACTAG12300.015.38211
GGTCTAG18900.014.9248541
GTCCTAG25250.014.5774121
TCTAGAC23700.013.9345033
GCTCTAG31200.013.67178251
GTCCTAA21200.013.6301761
CCGATCG2304.396074E-513.4605979
TAGTGCG5001.8189894E-1213.35646457
GTATAGG15200.013.3526191
GTACTAA19000.013.2168291
CTAGACT23000.013.162114
CTATACT23800.013.1533144
GCGCTAG3406.3528205E-813.1529281
TCTATAC21850.013.0675833
GTACATG39550.013.0467641
GTATTAA26900.012.7880931
Produced by FastQC (version 0.11.5)