FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11869860
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG29700.016.8171161
GTACTAA21000.015.2546981
GTATTAG23100.015.2099631
GTATAGG19600.015.1141021
GTTCTAG34150.015.0291871
TAGTGCG6500.014.57745857
GCTCTAA32300.013.9703961
CGATCGT5700.013.90263838
GTATATA37400.013.8152781
GTGCTAG28200.013.8028681
GTATTAA34350.013.6380441
TAGACTG35100.013.5441645
TCTAGAC27350.013.3508973
GTCCTAA24800.013.333971
GTTCTAA33350.013.32397651
TATACTG32650.013.1888535
GTACTAT20700.013.1460931
GTCTTAA31850.013.086271
TAGTCGG8700.013.066429
GTACATA32800.013.0223031
Produced by FastQC (version 0.11.5)