FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11869860
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA37100.017.8840941
GTATTAG21500.017.5197411
GTACTAG14500.016.9211561
GTCCTAG27300.016.5824361
GCTCTAG32200.016.2054371
GTCTTAG26150.015.990181
GGTCTAA22950.015.9611011
GTATAGG18650.015.0087951
GTCCTAA22800.014.8535711
GTATATA40000.014.2548811
GTACTAA21350.014.2437531
GTGCTAG29500.013.9400591
GTCTTAA33300.013.9059351
GTTCTAG32000.013.7149241
TCTATAC22600.013.2791183
CTAAGCG8050.013.2759614
GCCCTAG31250.013.2699981
GTACATA28150.013.1354521
TCTAGAC25750.013.12826253
CTATACT24600.013.0331084
Produced by FastQC (version 0.11.5)