FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12042293
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13950.016.5477091
GTATTAA32500.016.2197951
GTATTAG21350.016.1376171
TCTATAC23600.015.4755323
GTGCTAG27500.015.4102491
GTCCTAA23800.015.3449751
GTCCTAG28750.015.3394361
ATAAGCG8700.014.88446856
GTTCTAG35200.014.3883811
TATACTG34200.014.3059195
CTATACT27550.014.2572234
GTATAGG19350.014.244471
GTATACG5100.014.1868641
TCTAGAC27350.014.1095243
GTATAAG23450.013.6639851
GTATATA40800.013.5957441
GTTCTAA32150.013.3957261
GTACTAA21900.013.3724841
CTAGACT25350.013.048034
GCTCTAG34100.012.9328081
Produced by FastQC (version 0.11.5)