FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12042293
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG29000.016.9194641
GTCTTAG25250.016.6953181
TAGTGCG6550.016.6003357
GTACTAA21250.016.585841
GTATTAG22850.016.180571
GTTCTAG32950.015.6252551
CGCGAGA7950.015.59972317
GTACTAG13800.015.2737971
GTTCTAA37150.015.0678911
GGTCTAA23700.014.8712711
GTGCTAG28000.014.4385671
GCTCTAG34250.013.92242051
TAGTCGG9800.013.3579829
GTATAGG19350.013.2143861
GTATTAA32500.013.1836181
CGAATCG5500.013.1534589
GTCTATA21300.013.1401911
GTATATA39050.013.0074621
GTACATA31350.013.0059081
GCCTTAG27400.012.9892161
Produced by FastQC (version 0.11.5)