FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11945731
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21650.018.4516051
GTGCTAG30150.016.3336331
TAGTGCG5850.015.60520957
GTCCTAG28950.015.5832041
GTTCTAA32300.015.4596461
GTTCTAG35350.015.1973991
CGATCGT4550.015.14080738
TATACTG36200.015.031955
ATAAGCG10100.014.9141256
GCTCTAG34450.014.8946771
GTCTTAG25850.014.7875591
GTATATA40700.014.6381351
GTACTAA23850.014.4392851
GTACTAG12200.014.3960861
GTATAGG19150.014.3865041
GTATTAA30550.014.2034351
CTATACT28700.013.7998854
GGTCTAA22250.013.7750781
TAAGCGT9700.013.75510857
GTGTTAG28900.013.4652591
Produced by FastQC (version 0.11.5)