FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14119221
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14900.017.8875891
GTCTTAG28400.016.9171311
GTCCTAG34000.016.709391
GGTCTAG27550.015.7842721
GTACTAA22950.015.1278421
GTATTAG23500.014.6245561
GCTCTAG39050.014.3688981
GTATTAA38300.014.1008861
TCTGACG144950.014.0523470
GTGCTAA31100.013.8697691
GTGCTAG32000.013.6988621
GTCCTAA30500.013.68269351
CTAGACT29050.013.4956154
GGTCTAA26650.013.2907251
GTCTTAA34300.013.1892491
GTATATA35800.013.1264181
TCTATAC26300.013.0479383
GTATAGG22450.012.9654091
GTTCTAA39000.012.9485861
GTCCTAT29650.012.8921791
Produced by FastQC (version 0.11.5)