FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13982772
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22000.016.3876971
GTCCTAG30750.016.2777461
GTATTAA33500.015.9863751
GTACTAA21800.015.4140971
GTACTAG13950.014.8040721
GTCTTAG27300.014.2319261
GTCTTAA32250.014.2182111
GTTCTAG34700.013.8195691
GTCCTAA26150.013.6531181
TAGTGCG7300.013.42445357
GTATATA35900.012.96762851
GTTCTAA35100.012.9640181
GTATAGG21050.012.8038921
TATACTG35900.012.7729465
GTACTAT24250.012.7020641
GTGCTAA30650.012.6763961
GTATATG34800.012.5728631
GATCTAG26150.012.4484311
GTACTAC24050.012.3710691
TTATACT39450.012.244564
Produced by FastQC (version 0.11.5)