FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13831782
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG20750.018.8927361
GTCCTAG30650.015.5311411
GTACTAG14250.015.4746421
GTTCTAG34750.014.6052231
GTATATG35000.014.6009051
GTATTAA34950.014.4214951
TACGCGA2707.458584E-714.2601422
GTGCTAG30650.014.1607471
GTCTTAG27400.013.6687311
GTACTAA23450.013.5829311
GTATATA35050.013.4815771
GGTCTAA22400.013.4383331
GTTCTAA34500.013.0877681
GTCTTAA33750.013.0674761
ACTATAC20750.012.9888973
GTATAGA27500.012.9826231
GGTCTAG24450.012.8842331
GCTCTAG38000.012.5270921
GTCCTAA27950.012.5231381
ATCTCCG177500.012.40259970
Produced by FastQC (version 0.11.5)