FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11956973
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13650.019.6573471
GTATAGG19750.016.372831
GTATTAG23200.015.5691281
GTCCTAG30250.015.4659461
TCTATAC26150.015.3913013
GCGCTAG4151.8189894E-1214.9206381
GTCCTAA25900.014.4773711
GTATTAA33800.014.4522221
GTACTAA21350.014.0179441
CTATACT29300.013.7366054
GTGCTAG32900.013.6974071
GTACAAG39550.013.5687891
TCAGTAC31250.013.4298443
GGTCTAA24450.013.0847991
CGATCGT3952.417437E-913.06834638
GTACTAT24250.013.0508581
CTAGTAC13500.012.9956373
GCTCTAG33900.012.9889261
TATACTG36200.012.8289715
GTCTTAA31800.012.7649131
Produced by FastQC (version 0.11.5)