FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11956973
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13850.017.4215581
GTGCTAG27550.017.1410621
GTCCTAG31100.016.2927991
GTACTAA22050.015.9452131
GTATTAG22550.015.5916611
GTATATA43500.015.3727371
GTACTAT24850.014.7034151
GTCTTAG27850.014.6047981
GTTCTAG36000.014.4581661
GTCCTAA27250.014.4203931
GTATTAA36350.014.2241261
GTGTTAG31450.014.0290441
GGTCTAG22900.013.6976051
GTATATG35950.013.615331
GGTCTAA25900.013.5749791
GCTCTAG34200.013.40492251
GGTTATA25650.013.3041331
CGAATCG4406.366463E-1113.29003059
GCGATCG4204.311005E-1013.108829537
GTGCTAA29250.013.0808471
Produced by FastQC (version 0.11.5)