FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11773180
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136620.11604341392894699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13400.018.0103361
CGATCGT4850.017.74231338
GCGATCG5250.017.04396637
GTATTAG25800.016.3030431
GTCCTAG31650.015.6861861
GTTCTAG33050.015.3346691
GTACTAA22200.015.2195441
GTATAGG19800.014.9748271
GTCCTAA24500.013.79077151
TATACTG33150.013.7013135
GTATATA44650.013.6672251
GTATTAA35350.013.4591681
CTAGACT25900.013.1587834
GTCTTAG25850.013.0705571
GTATATG36150.013.0659451
GCTCTAG35950.013.0427321
GTACAGG49450.012.8983441
GTTCTAA32450.012.85580351
GTGCTAG32900.012.7847571
GTACTAT23250.012.7527561
Produced by FastQC (version 0.11.5)