FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11865210
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14900.016.8572121
GTATTAG25200.016.3843751
GTATTAA32450.015.9047081
GTCCTAG31000.015.7607131
GTACTAA23900.015.5480181
GTGCTAG28400.015.1440091
GTCTTAG28350.014.9279861
GTATATA42700.014.5847791
GGTCTAG23700.014.2274451
GTATACG5050.013.6266091
GTACTAT22200.013.4838971
GTTCTAG33000.013.4500821
GTCCTAA24450.013.3688461
GTTCTAA32750.013.3426341
GTACATA31600.012.9571371
TATACTG35050.012.8535155
GTATAGG19400.012.7696771
GTACAGG50400.012.62962151
GGTCTAA21100.012.5561771
GTGTTAG31900.012.51170351
Produced by FastQC (version 0.11.5)