FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11803586
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125390.10623042861720158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15150.018.1642481
GTATTAG25050.017.0276221
GTCCTAG31250.016.5113031
GTGCTAG30250.015.4651311
GTTCTAA32750.014.8097541
GTTCTAG34800.014.6292671
GTACTAA22900.014.5705621
GTATTAA33350.014.5433121
GTCCTAA25100.014.389831
CCTATAC19000.013.9368013
GTATATA42650.013.7110251
GTCTTAG26650.013.68197251
CTATACT26450.013.1316114
GCCCTAG31400.013.036391
GTGCTAA27500.013.0089041
GTATTAT34100.012.9120631
GGTCTAA22650.012.9089461
TCTATAC23600.012.8232063
TATACTG32650.012.63981155
GTATAGG19100.012.4266991
Produced by FastQC (version 0.11.5)