FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10453553
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108850.10412727615194567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG25200.017.4393141
GTATTAG18900.016.844271
GTACTAG11500.015.6470231
GTGCTAG26900.015.1794471
GTACTAA18800.014.9091661
TCTATAC22050.014.2599453
GTATAGG17250.014.2428031
GTCTTAG23100.014.2310791
GTCCTAT23550.013.9591471
TACGCGA2253.4072706E-513.830322
GTCCTAA22150.013.5916371
CTATACT23150.013.5716424
GGTCTAA19600.013.4178741
GTTCTAG28900.013.4105441
GTATTAA31900.013.2341281
GGTCTAG22000.013.0551421
GTCTTAA28950.012.9092611
GTACATA25550.012.8664551
GTATACG3508.589086E-812.8529121
GTTCTAA30300.012.676711
Produced by FastQC (version 0.11.5)