FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10553453
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12750.016.7807311
GTCCTAG25150.016.7398361
GTACTAA19350.015.3372291
GTATTAG19500.015.2192491
GTCCTAA23200.015.0232111
GGTCTAA19650.014.576221
GTACTAT20850.013.7373021
GTATTAA32250.013.5894981
GTATAGG15800.013.3230031
GTGCTAG27150.012.9646181
GTACATA28300.012.9255431
GTATATA35550.012.9104521
GTCTAGA22950.012.6306581
GTTCTAG29950.012.4438941
GTATAAG21050.012.2952841
ATTAGAC22650.012.1884683
TCTAGAC21950.012.1055223
GTCTTAG22900.011.9047691
GTCTTAA28850.011.8418281
GTGCTAA24950.011.7564921
Produced by FastQC (version 0.11.5)