FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10340307
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG23950.017.0045931
GTACTAA17500.016.7637271
GTACTAG10850.015.268671
GTATTAG19800.014.9907371
GGTCTAA19900.014.7419711
GTATATA33650.014.4618471
GTTCTAG27050.013.9074961
GTGCTAG23650.013.8637951
GTATTAA30400.013.6237731
GTCTTAG24800.013.2209191
TCTATAC19250.012.7299213
GTATTAT29150.012.4319831
GTCCTAA22950.012.1812551
GTACAGA46350.011.6906771
GTGCTAA25450.011.6627351
GTATACG4451.3604222E-811.6337831
GTGTTAG26450.011.6132571
GCGCTAA3903.6306847E-711.5045191
GCCTTAG23100.011.50451851
TCTAGAC21950.011.4785353
Produced by FastQC (version 0.11.5)